Product for the preparation of cyto-histological samples for optical microscopy.
Rapid staining method for frozen sections (thickness 6-8 micron).
WARNING
If fresh tissue have been fixed in formalin or other fixative, it is necessary to rinse well in distilled water before staining in hematoxylin.
-For section dehydration and clearing the procedure requests: 2 changes 95° ethyl alcohol, 2 changes absolute ethyl alcohol, 2 changes of xylene.
METHOD
Preparation of buffer solution: In a 100 ml, jar put 10 drops of reagent B. The kit allows making 100 ready to use buffer solutions of 100 ml each.
Put the section in the REAGENT A, 45-60 seconds
Buffer solution, 5 dips
Rinse in tap water, 5 dips
Put the section in the REAGENT C, 30 seconds
Ethanol 95°, 5 dips
Absolute ethanol, 5 dips
Xylene, 10 dips
Mount in synthetic mounting medium
Product classification
The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform Bio-Optica Milano S.p.A and the competent authorities.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.