Minimum number of tests that can be performed 100
Completion time 30 minutes
Shelf life 2 years
Additional equipment Not required
Application
Identification of elastic fibers on tissue sections
Result
Elastic fibers from dark brown to dark purple
Background almost colorless
Product for the préparation of cyto-histological samples for optical microscopy.
To demonstrate eiastic fibres in tissue sections. Recommended for vascular pathology.
METHOD
1) - Bring section to distiiled water.
2) - Put on the section 5 drops of reagent A and 5 drops of reagent B: leave to act 4 minutes.
3) - Wash the slide in distiiled water.
4) - Put on the section 10 drops of reagent C: leave to act 1 minute (until being colourless).
5) - Double washing in distiiled water.
6) - Préparé the incubation box: put on the bottom of the incubation 20 drops of reagent D; introduce the slide and put on the section 10 drops of reagent E. Close the incubation box and incubate 20 minutes.
7) - Wash well in distiiled water.
8) - Put on the section 10 drops of reagent F: leave to act 2 minutes.
9) - Rinse in running water 1 minute.
10) - Dehydrate through ascending alcohols, clear in xylene and mount.