Staining solution reagent Enzymatic digestion hyaluronidase

for histologyfor cytology

Minimum number of tests that can be performed 10 Completion time 10 minutes Shelf life 1 year Storage conditions 2-8°C Additional equipment 100 ml vertical jar, oven Application The digestion of histological sections with hyaluronidase is indicated for removing the following complex carbohydrates: hyaluronic acid and chondroitin sulfate A and B. Result The absence of staining with Alcian Blue detectable on the sample section is attributable to the presence of hyaluronic acid or chondroitin sulfates. Method 1) Bring two serial sections to the water (one sample section and one control section). 2) Prepare the hyaluronidase solution: pour the entire contents of bottle B into bottle A (you are advised to set aside a portion of buffer A and use it for washing bottle B after the transfer of the enzyme.) Stir vigorously until completely dissolved. Transfer the resulting solution to a vertical histology jar. Place the sample section in it. 3) Transfer the entire contents of bottle C to a vertical histology jar. Place the control section in it. 4) Incubate both sections for one hour at 37°C. 5) Wash both slides in running water for 5 minutes. 6) Stain with Alcian Blue.