Product for the preparation of cyto-histological samples for optical microscopy.
Recommended method for connective tissue, especially to differentiate collagen fibres from connective tissue.
PRINCIPLE
Three different dyes are used: Weigert’s iron hematoxylin for nuclei, picric acid for cytoplasm, and acid fuchsin for collagen.
METHOD
1) Bring section to distilled water.
2) Put on the section 5 drops of reagent A and add 5 drops of reagent B: leave to act 10 minutes.
3) Blue in running tap water for 10 minutes.
4) Put on the section 10 drops of reagent C: leave to act 10 minutes.
5) Wash rapidly (2-3 seconds) in distilled water and dehydrate quickly through ascending alcohols, stop 1 minute in the last absolute ethanol, clear in xylene and mount.
Product classification
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.
In the event of a serious accident, we recommended that you immediately inform Bio-Optica Milano S.p.A and the competent authorities.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.