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Stain reagent Colloidal iron
staining solutionfor cytologyfor histology

Stain reagent - Colloidal iron - BIO-OPTICA Milano - staining solution / for cytology / for histology

Stain reagent - Colloidal iron - BIO-OPTICA Milano - staining solution / for cytology / for histology

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Go to the BIO-OPTICA Milano website for more information

Characteristics

Type

staining solution, stain

Applications

for histology, for histopathology, pathology, for cytology

Storage temperature

Min.: 15 °C
(59 °F)

Max.: 25 °C
(77 °F)

Description

Product
Colloidal iron reagent for diagnostic/cytohistological staining. Pack for 100 tests.

Product code & pack
04-180809 · Pack: 100 tests

Documents available
Technical datasheet languages

IT
EN
CS
DK
DE
EL
ES
ET
FI
FR
HR
HU
LT
LV
NL
PL
PT
RO
SK
SE
BG

Safety datasheet (SDS) languages

IT
EN
DA
DE
EL
ES
ET
FI
FR
HU
LT
LV
NL
PL
PT
RM
SK
SE
NO
BL
CR
CZ
RU
TR

CE conformity
IT, EN

Characteristics / technical specifications

Commercial name: Colloidal iron
Product code: 04-180809
Pack: 100 tests
Document types: Technical datasheet (multilanguage), Safety datasheet / SDS (multilanguage), CE conformity
Manufacturer / Brand: Bio-Optica

Intended use & complementary equipment

Intended for the detection of acidic mucins (sialomucins and sulfomucins) in cyto‑histological preparations for optical microscopy.
Minimum number of tests: 100
Typical procedure time: approx. 1 h 35 min
Product shelf life: 2 years
Recommended complementary equipment: vertical histology staining trough 50 ml, graduated cylinder, glass stirring rod

Application & specificity
Method indicated for visualization of acidic mucins. The reaction highlights acidic mucins (sialomucins and sulfomucins) whose acidic groups, at the reaction pH, are in an anionic form and can form a stable complex with trivalent ferric ions.

Principle
The method is based on the interaction, at strongly acidic pH, between colloidal iron and the acidic groups of the mucopolysaccharide chain. Under these conditions iron forms a chelate detectable by the Prussian blue reaction: potassium ferrocyanide reacts in acidic environment with the ferric ions of the chelate to form an insoluble colored salt (Prussian blue). The ionic reaction occurs as follows:

4 Fe++ + 3 K4Fe(CN)6 = Fe4Fe(CN)6)3 + 12 K+

Results

Acidic mucins: blue
Cell nuclei: red

Manufacturer
Bio-Optica

Catalogs

General Catalogue

General Catalogue

139 Pages

Go to the BIO-OPTICA Milano website for more information

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