Staining solution reagent P.T.A.H.

for cytologyfor histology

Product for the preparation of cyto-histological samples for optical microscopy. To demonstrate elastic fibres in tissue sections. PRINCIPLE The method is based on the affinity towards elastic fibres displayed by resorcin fuchsin, a precipitate resulting from a reaction between resorcin, basic fuchsin and ferric chloride. Since this is not a specific method, other structures such as collagen and basal membranes might be stained. Therefore, it is essential to differentiate carefully in order to obtain a selective marked staining of elastic fibres. Weigert’s “long method” differs from “quick method” in both staining mixture and procedure; it is more selective because staining mixture is less concentrated and incubation times are very long. METHOD 1) Bring section to distilled water. 2) Put on the section 5 drops of reagent A and add 5 drops of reagent B: leave to act for 5 minutes. 3) Rinse in distilled water. 4) Put on the section 10 drops of reagent C, leave to act for 5 minutes. 5) Rinse in distilled water. 6) Transfer the reagent D in a Coplin jar, introduce the slide, cap the jar and incubate overnight. After use, in order to reduce the ethanol evaporation, it is recommended to re-introduce the solution in the original vial. 7) Rinse in distilled water. Put on the section 10 drops of reagent E: leave to act 10 minutes. 8) Rinse well in distilled water. 9) Dehydrate through ascending alcohols, clear in xylene and mount. Product classification The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases)