Staining solution reagent Afog
for histologyfor cytology

staining solution reagent
staining solution reagent
Add to favorites
Compare this product
 

Characteristics

Type
staining solution
Applications
for histology, for cytology

Description

Minimum number of tests that can be performed 100 Completion time 22 minutes Shelf life 2 years Storage conditions 15-25°C Additional equipment Not required Application Reference method for highlighting protein deposits in renal biopsy. Recommended fixative: Bouin. Result Collagen fibrils blue Nuclei black Erythrocytes, cytoplasm pink - orange Elastic fibers pale pink - yellow or colorless Protein deposits bright red Product for the préparation of cyto-histological samples for optical microscopy. Standard procedure for visualisation of glomerular protein deposits in kidney biopsy; recommended fixative: BOUIN PRINCIPLE In this method, three different dyes are used: Weigert hematoxylin for nuclear staining, orange G for cytoplasm and aniline blue for a sélective collagen staining. Selectivity in this procedure is due to different degrees of affinity between dyes and tissue macromolecules. A central rôle is played by phosphomolibdic acid, which acts as a bound between tissue structures (collagen fibrils, cell membranes), and aniline blue (amphoteric dye). Orange G, which is the second component of AFOG solution, has no affinity to phosphomolibdic acid and is thus used to stain ail remaining structures. Acid fuchsin stains selectively glomerular protein deposits.

Catalogs

No catalogs are available for this product.

See all of BIO-OPTICA Milano‘s catalogs

Other BIO-OPTICA Milano products

Staining kits

*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.