Stain reagent Afog
staining solutionfor histologyfor histopathology

Stain reagent - Afog - BIO-OPTICA Milano - staining solution / for histology / for histopathology
Stain reagent - Afog - BIO-OPTICA Milano - staining solution / for histology / for histopathology
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Characteristics

Type
staining solution, stain
Applications
for histology, tissue, for histopathology, pathology, laboratory
Format
liquid
Method
chromogenic, colorimetric
Storage temperature

Min.: 15 °C
(59 °F)

Max.: 25 °C
(77 °F)

Description

Description
Product code: 04-021002
Commercial name: Afog
Pack size: 100 tests
Minimum number of tests: 100
Processing time: 22 minutes
Shelf life: 2 years
Additional equipment: Not required
Intended use: For professional laboratory use by healthcare professionals. The product is classified as hazardous; read the label and safety data sheet before use.

Application and principle
Reference method for demonstrating protein deposits in renal biopsy. Recommended fixative: Bouin.
  • Principle: nuclear staining with ferric hematoxylin, cytoplasmic staining with Orange G and highly selective collagen staining with aniline blue mediated by phosphomolybdic acid.
  • Orange G does not bind phosphomolybdic acid and stains the remaining structures.
  • Acid fuchsin highlights protein deposits in bright red.

  • Results: collagen fibrils — blue
  • nuclei — black
  • erythrocytes, cytoplasm — pink-orange
  • elastic fibers — pale pink-yellow or colorless
  • protein deposits — bright red


Method (overview)
  • Hydrate section and apply reagents A and B (5 drops each) for 10 minutes, rinse.
  • Apply reagent C (10 drops) for 5 minutes, rinse; apply reagent D (10 drops) for 5 minutes, rinse.
  • Dehydrate, clear and mount. See technical datasheet for full protocol and reagent composition.


Documents available
  • Technical datasheet: IT, EN, CS, DK, DE, EL, ES, ET, FI, FR, HR, HU, LT, LV, NL, PL, PT, RO, SK, SE, BG
  • Safety datasheet (SDS): IT, EN, DK, DE, EL, ES, ET, FI, FR, HU, LT, LV, NL, PL, PT, RM, SK, SE, NO, BL, CR, CZ, RU, TR
  • CE conformity: IT, EN


Safety
The product is intended for professional laboratory use by healthcare professionals. The product is classified as hazardous. Read the label (hazard symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.

Technical specifications
  • Product code: 04-021002
  • Commercial name: Afog
  • Pack size: 100 tests
  • Documents: Technical datasheet and SDS in multiple languages; CE conformity certificate

Catalogs

Other BIO-OPTICA Milano products

Special Stains

*Prices are pre-tax. They exclude delivery charges and customs duties and do not include additional charges for installation or activation options. Prices are indicative only and may vary by country, with changes to the cost of raw materials and exchange rates.