Stain reagent Picrosirius Red

staining solutioncollagenfor cytology

Product for the preparation of cyto-histological samples for optical microscopy. For demonstration of collagen fibres and bilirubin in tissue sections. PRINCIPLE Sirius red picrate solution performs a double function: - to stain collagen by the binding between Sirius red and some proteins containing basic amino acids - to stain bilirubin by conjugation by picric acid Method 1) Bring the section to the distilled water. 2) Dispense 10 drops of reagent A onto the section: leave to act for 50 minutes. 3) Wash briefly in distilled water. 4) Dispense 10 drops of reagent B onto the section: leave to act for 2 minutes. Repeat twice. 5) Wash briefly in distilled water and drain the slide. 6) Dispense 10 drops of reagent C onto the section: leave to act for 3 minutes. 7) Leave to develop in tap water: 3 minutes. 8) Wash in distilled water and dehydrate rapidly by means of the ascending series of alcohols, leaving for 1 minute in the last absolute: xylene and balsam. Warning Product classification The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform Bio-Optica Milano S.p.A and the competent authorities. Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal. Stability After the first opening, the product is reusable until the expiry date, if correctly stored.