Staining solution reagent Congo Red

for cytologyfor histology

Product for the preparation of cyto-histological samples for optical microscopy. Method to show amyloid in tissue sections. PRINCIPLE This is an empirical method. It is still doubtful whether the formation of the link between amyloid and dye is due to proteins or to glycan in amyloid, or even to both. On the contrary, it is certain that amyloid stained with Congo red displays a marked birefringence in polarized light. From this anisotropy, it has been inferred that amyloid may have an intrinsic molecular organization that is emphasized by Congo red. According to Wirchow and Puchtler, amyloid is the only component in human tissues whose histochemical characteristics can be compared to those of cellulose; therefore, it has been supposed that dye and amyloid are linked by hydrogen bonds, just as it happens with the link between cellulose and Congo red. METHOD 1) Bring section to distilled water. 2) Put on the section 10 drops of reagent A: leave to act 15 minutes. 3) Rinse in distilled water. 4) Put on the section 10 drops of reagent B: leave to act 30 seconds. 5) Wash 5 minutes in running tap water. 6) Put on the section 10 drops of reagent C: leave to act 2 minutes. 7) Put on the section 10 drops of reagent D: leave to act 5 minutes. 8) Blue in running tap water 5 minutes. 9) Dehydrate through ascending alcohols, clear in xylene and mount. Product classification The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged.