Minimum number of tests that can be performed 100
Completion time 25 minutes
Shelf life 2 years
Storage conditions 15-25°C
Additional equipment Glass histology jar with lid
Application
Method indicated for highlighting lipids on cryostat sections of tissue having a thickness
of 5 μm.
Fixation: you are advised to use saline formalin or Baker fixative in order to make the
phospholipids less soluble.
Result
Fatty acids bright red
Nuclei blue
Product for the préparation of cyto-histological samples for optical microscopy.
Recommended method for démonstration of lipids on 5 pm frozen sections.
Fixation: it is recommended the use of Saline formalin or Baker1 s fixative in order to maintain the phospholipids.
PRINCIPLE
Oil red O solution stains selectively cellular lipids while hematoxylin solution stains nuclei.