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Solution reagent AgNOR
staining solutionstainFFPE tissues

Solution reagent - AgNOR - BIO-OPTICA Milano - staining solution / stain / FFPE tissues

Solution reagent - AgNOR - BIO-OPTICA Milano - staining solution / stain / FFPE tissues

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Go to the BIO-OPTICA Milano website for more information

Characteristics

Type

solution, staining solution, stain

Applications

FFPE tissues, for microscopy, pathology, for histopathology, for histology, for cytology

Format

liquid

Storage temperature

Max.: 25 °C
(77 °F)

Min.: 15 °C
(59 °F)

Volume

10 ml
(0.34 US fl oz)

Description

Product overview
Staining kit for detection of AgNOR (argyrophilic nucleolar organizer region) proteins (100 kD) in paraffin-embedded sections and cytology smears. Reference: 04-045801. Supplied for 48 tests. Minimum number of preparations: 12 (up to 4 slides per preparation). Total processing time per run: 30 minutes.

Applications

Histopathology and cytology laboratories for visualization of nucleolar organizer region (NOR) proteins by light microscopy.
Used on paraffin-embedded tissue sections and air-dried / fixed smears.

Principle
AgNOR proteins (100 kD) act as reducing agents that bind silver ions and produce a metallic silver deposit visible under optical microscopy. Immediately before use, silver nitrate solution is combined with a positively charged hydrocolloid (e.g., gelatin) to enhance contact between reducing groups of nucleolar proteins and silver ions, resulting in argyrophilic staining of NOR-associated proteins.

Contents & storage

Kit size: 48 tests (minimum 12 preparations × up to 4 slides each).
Shelf life: 1 year.
Recommended storage: 15–25°C.
Additional equipment required: glass rod, staining jars/wash containers, slide racks (non-metallic recommended).

Method (summary)

Hydrate section or smear with distilled water.
Prepare working solution: pour entire vial A into the slide container and add 10 ml of reagent B; mix gently with a clean glass rod previously rinsed in distilled water.
Incubate sections/slides in working solution in the dark for 30 minutes at room temperature.
Rinse thoroughly in three changes of high-quality distilled water.
Add 10 drops of reagent C to the section/slides and incubate for 1 minute.
Rinse in distilled water, dehydrate through ascending alcohols, clear in xylene and mount with appropriate mounting medium.

Warnings

Use only high-quality distilled water for all washes.
Do not use poly-lysine-coated slides.
Avoid contact with metal objects (tweezers, metal racks) during staining.
After mounting, store slides protected from light.
If reagent A is found gelled due to low-temperature storage, restore fluidity by warming the bottle under warm running water before use.

Catalogs

General Catalogue

General Catalogue

139 Pages

Go to the BIO-OPTICA Milano website for more information

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