Product for the preparation of cyto-histological samples for optical microscopy.
Application
Cryostatic sections of 8 microns of human skeletal muscle. Histochemical demonstration of phosphorylase.
Method
1) Put restoration solution (reagent B) into lyophilized powder (reagent A).
2) Shake until dissolution.
3) Incubate sections in rehydrated phosphorylase solution at 37°C, 60 minutes.
4) Dip quickly in alcohol 50%, 30 seconds.
5) Fix in absolute alcohol, 15 minutes
6) Develop in Lugol solution 10%, 2 minutes.
7) Mount in glycerine containing a drop of concentrated Lugol.
Results Phosphorylase enzymes activity: various tones of blue
Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk, and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.