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Staining solution reagent 30-30121LY
for histology

Staining solution reagent - 30-30121LY - BIO-OPTICA Milano - for histology

Staining solution reagent - 30-30121LY - BIO-OPTICA Milano - for histology

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Characteristics

Type: staining solution
Applications: for histology
Storage temperature: -20 °C
(-4 °F)

Description

Product for the preparation of cyto-histological samples for optical microscopy. Application Cryostatic sections of 8 microns of human skeletal muscle. Valuation of the alkaline phosphatase activity. Alkaline phosphatase is particularly useful in revealing sites of phagocytosis and inflammation in muscle biopsy specimens. Method 1) Put restoration solution (reagent C) into lyophilized powder (reagent A). 2) Shake until dissolution. 3) Add the reconstituted reagent A solution to the reagent B. Shake until dissolution. 4) Incubate sections in the obtained solution (A+B+C) at 37°C for 1 hour. 5) Wash in distilled water 6) Fix in 10% formalin, 10 minutes 7) Wash in tap water, 5 minutes 8) Mount with aqueous mounting medium. We recommend using the working solution (step 4 of the method) just prepared and discard after use. Results Positive alkaline phosphatase enzymatic activity: black Background: ochre yellow Warning and precaution Storage Stability Disposal REVISION N° The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk, and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities. Disposal Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.

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