Staining solution reagent 30-30125LY

for histology

Product for the preparation of cyto-histological samples for optical microscopy. Application Cryostatic sections of 8 microns of human skeletal muscle. Typing of the muscle fibres. Method The ready for use solutions provided allow the method to be run simultaneously on three serial sections of the sample to be examined. The reaction can be performed using the reagent containers as the incubation dish. Alternatively, the reaction can be performed using vertical dishes with a capacity of 50 ml positioning the section in the lower part of the slide. It is recommended to let the reagents reach room temperature before starting the procedure. 1) The staining jars should be marked with 4.3 - 4.7- 10.4. Sections must be marked with the same number. 2) Incubating medium preparation (reagents 4 and 5): put about 10 ml of reagent 5 (restoration solution) into the lyophilized powder (reagent 4). Shake until dissolution and add the obtained solution to reagent 5 container to obtain 30 of incubating medium. 3) Introduce the 4.3 slide into the reagent 1 container. Incubate the 4.3 section at 37°C, 10 minutes. 4) Introduce the 4.7 slide into the reagent 2 container. Incubate the 4.7 section at 37°C, 10 minutes. 5) Introduce the 10.4 slide into the reagent 10.4 container. Incubate the 10.4 section at 37°C, 10 minutes. 6) Drip the slides. 7) Put the sections into the 5 containers (incubating medium). Incubate at 37°C 30 minutes. 8) Wash the sections with distilled water. 9) Put the sections into the reagent 6 containers. Incubate 2 minutes. 10) Repeat the step using the second change of reagent 6. 11) Wash well with distilled water.