Product for the preparation of cyto-histological samples for optical microscopy.
Application
Cryostatic sections of 8 micron of human skeletal muscle. To show PFK enzymatic activity. This procedure is used to determine if a glycogen-storage disease is due to a lack of PFK activity or about other enzymes necessary for the metabolism of glycogen.
Method
1) Put restoration solution (reagent B) into lyophilized powder (reagent A).
2) Shake until dissolution.
3) Put the sections horizontally.
4) Cover the sections with right quantity of rehydrated PFK solution.
5) Incubate sections in the incubation box at 37°C 60 minutes
6) Rinse in distilled water 3 changes
7) Dehydrate in acetone 30, 60, 90%
8) Dehydrate in absolute ethanol, clear and mount.
Warning and precaution
The product is intended for professional laboratory use for healthcare professionals.
Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.