Product for the preparation of cyto-histological samples for optical microscopy.
Application
Cryostatic sections of 8 micron of human skeletal muscle.
Valuation of the succinic dehydrogenase activity specifically in mitochondria.
Method
1) Put restoration solution (reagent B) into lyophilized powder (reagent A).
2) Shake until dissolution.
3) Incubate sections in rehydrated SDH solution at 37°C, 45 minutes
4) Rinse in distilled water 3 changes
5) Fix in neutral formalin 10%, 10 minutes
6) Place in 15% ethanol, 10 minutes
7) Mount with aqueous mounting medium.
Results Positive SDH activity : grey-blue
Warning and precaution The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.
Disposal
Hazardous preparation: observe all state and local environmental regulations regarding waste disposal.