Product for the preparation of cyto-histological samples for optical microscopy.
Application Cryostatic sections of 8 microns of human skeletal muscle. To show the enzymatic activity of Myoadenylate deaminase.
Method
1) Put restoration solution reagent B into lyophilized powder (reagent A).
2) Shake until dissolution.
3) Put NBT solution (reagent C) into the reconstituted solution A
4) Shake well.
5) Stain the sections in the obtained solution (A+B+C) at room temperature for 1 hour.
6) Rinse in distilled water.
7) Mount in glycerine jelly.
Results
Positive Myoadenylate deaminase enzymatic activity: blue
Warning and precaution
The product is intended for professional laboratory use for healthcare professionals. Carefully read the information on the label (danger symbols, risk, and safety phrases) and always consult the safety data sheet. Do not use if the primary container is damaged. In the event of a serious accident, we recommended that you immediately inform BioOptica Milano S.p.A and the competent authorities.